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目的观察亚低温干预对犬心脏骤停后脑水肿及血脑屏障的影响。方法共选取16只成年健康杂种犬,将其随机分为亚低温组(n=8)和对照组(n=8),采用诱发室颤的方法导致上述2组动物心跳、呼吸骤停,随后施行脑复苏程序;亚低温组动物在心跳骤停期间给予亚低温干预。采用双抗夹心酶联免疫吸附技术测定各组动物血清S100B蛋白含量,同时观察其脑组织含水量及病理学改变。结果亚低温组动物经亚低温干预后,发现其血清S100B蛋白含量显著低于对照组(P<0．05),脑组织含水量也显著低于对照组(P <0．05);2组实验动物脑标本经病理学检测后发现,亚低温组动物脑组织缺氧损伤程度明显轻于对照组。结论亚低温干预能减轻心跳骤停实验犬的脑水肿程度,改善其血脑屏障功能,从而发挥脑保护效应。
Objective To observe the effects of mild hypothermia on cerebral edema and blood-brain barrier after cardiac arrest in dogs. Methods A total of 16 adult healthy mongrel dogs were selected and randomly divided into mild hypothermia group (n = 8) and control group (n = 8). The induced VF was used to induce heartbeat and respiratory arrest in the above two groups of animals. Cerebral resuscitation procedures were performed. Mild hypothermia interventions were given to animals in the hypothermia group during cardiac arrest. Serum levels of S100B protein in each group were measured by double-antibody sandwich enzyme-linked immunosorbent assay (ELISA), and their brain water content and pathological changes were also observed. Results After mild hypothermia, the level of S100B protein in the mild hypothermia group was significantly lower than that in the control group (P <0.05), and the brain water content was also significantly lower than that in the control group (P <0.05) The pathological examination of brain samples of experimental animals showed that hypoxia injury in brain tissue of mild hypothermia group was significantly lighter than that of control group. Conclusion Mild hypothermia can reduce cerebral edema in dogs with cardiac arrest and improve the function of blood-brain barrier, so as to exert cerebral protection effect.