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目的了解马鞍山市不同来源副溶血弧菌分离株的病原学及分子生物学特征。方法从市场流通食物、食物中毒事件标本及临床腹泻病例标本中分离培养、鉴定副溶血弧菌;并进行尿素酶、神奈川试验、药敏试验;PCR方法检测tlh、tdh和trh基因;脉冲场凝胶电泳(PFGE)进行分子分型。结果 40株不同来源的副溶血弧菌尿素酶阳性17株,神奈川试验阳性20株;头孢唑啉的耐药达到95.00%,氨苄西林的耐药达到69.00%,对阿米卡星、头孢哌酮/舒巴坦、头孢他啶、头孢吡肟、头孢噻肟、庆大霉素、亚胺培南、左氧氟沙星、派拉西林/三唑巴坦、美洛培南均100.00%敏感;毒力基因检测tlh阳性36株,阳性率为90.00%,tdh 29株阳性,阳性率为72.50%,40株菌trh全部阴性;PFGE将40株副溶血弧菌分成19种型别,部分菌株相似性100.00%,整体菌株带型相对分散。结论马鞍山市副溶血性弧菌对大多数抗生素均具有较高敏感性,3种毒力基因携带率低,PFGE提示有部分菌株有流行趋势,但整体没有发现明显优势菌群,提示本地食品中副溶血性弧菌未出现大流行迹象。
Objective To understand the etiological and molecular biological characteristics of Vibrio parahaemolyticus isolates from different sources in Ma’anshan City. Methods Vibrio parahaemolyticus was isolated and cultured from circulating food, food poisoning incidents and clinical diarrhea specimens. The urease, Kanagawa test and drug susceptibility test were performed. The tlh, tdh and trh genes were detected by PCR. Gel electrophoresis (PFGE) molecular typing. Results 40 strains of Vibrio parahaemolyticus were positive for 17 strains of urease and 20 for Kanagawa. The resistance to cefazolin was 95.00% and the resistance to ampicillin was 69.00%. The resistance to amikacin, cefoperazone / Sulbactam, ceftazidime, cefepime, cefotaxime, gentamicin, imipenem, levofloxacin, paracetamol / tazobactam, meropenem were 100.00% sensitive; virulence gene detection tlh 36 strains positive, 90.00% positive, tdh 29 positive, the positive rate was 72.50%, 40 strains of bacteria trh all negative; PFGE 40 strains were divided into 19 types of Vibrio parahaemolyticus, part of the similarity of 100.00% Strains with relatively dispersed strains. Conclusion Vibrio parahaemolyticus in Ma’anshan City is highly sensitive to most of the antibiotics. The three virulence genes are low in carrying rate. PFGE suggests that some strains have epidemic tendency. However, no obvious predominant flora is found in the whole population, suggesting that Vibrio parahaemolyticus showed no signs of pandemic.