黄瓜雌性杂种已广泛应用于商品生产,在腌制黄瓜中,雌性杂种的比例相当大。为了培育雌性近交系,繁育雌性杂种,单株选系必须采取无性繁殖,以便为近交提供足够的植株。现行的方法是将3～4根插条固定在含有吲哚丁酸(1、2、3)的充汽液中,成苗定植后,至少要在一个植株上,用赤霉酸诱导出雄花,为繁殖雌性选系提供花粉。然而,这种方法幼苗有时不生根,或者死去,移栽到土里时,也特别难成活。当一株苗只能截取3～4个插条,而且至少要存活两个插条(一个雌的,一个雄的),以维持单株选系时,这一问题就变得更加突出。因此,从一个雌性选系植株中能繁殖许多苗的组织培养法是令人向往的。以前,尽管曾经报导过南瓜的愈伤 Cucumber female hybrids have been widely used in commercial production, in pickled cucumbers, the proportion of female hybrids is quite large. In order to breed female inbreds and to breed female hybrids, individual lines must be cloned in order to provide sufficient plants for inbreeding. The current method is to fix 3 to 4 cuttings in a steam-filled liquid containing indole butyric acid (1, 2, 3). After the seedlings are planted, at least one plant must be used to induce male flowers with gibberellic acid, Provide pollen to breeding female lines. However, seedlings of this method sometimes do not root or die, and are particularly difficult to survive when transplanted into the soil. This problem becomes even more pronounced when a seedling can only intercept 3 to 4 cuttings and at least two cuttings (one female and one male) survive in order to maintain a single plant selection. Therefore, the tissue culture method that can multiply many shoots from one female selected line plant is desirable. In the past, although pumpkin calluses have been reported