洋地黄中毒在临床上颇为常见,中毒发生率约占洋地黄化患者的10～20%。过云由于缺乏特异的对抗剂,对洋地黄中毒的治疗主要是以对症治疗为主,效果不佳,死亡率达5～10%。Butler 等自1967开始用地高辛与白蛋白交联后免疫动物而得到抗地高辛抗体,并制备了其 Fab片段,在实验动物体内或体外均可特异地与洋地黄结合使之失活,引起了人们的注意。1976年 Smith 第一次应用此 Fab 片段抢救一例服用大剂量洋地黄严重中毒患者获得成功。自此,用此抗体片段已救治了许多洋地黄中毒患者。实验方法与结果一.体外温育的解毒作用健康小白鼠,体重24±2g,测得腹腔注 Digitalis poisoning is quite common in clinical practice, the incidence of poisoning accounts for about 10 to 20% of patients with digitalis. Over the cloud because of the lack of specific antagonists, the treatment of digitalis poisoning is mainly based on symptomatic treatment, the effect is poor, the mortality rate of 5 to 10%. Butler et al. Have been used to immunize animals after cross-linking of digoxin with albumin from 1967 to obtain anti-digoxin antibodies, and Fab fragments thereof are prepared and can be specifically combined with digitalis to inactivate them in vivo or in vitro. Aroused people’s attention. In 1976, Smith first applied this Fab fragment to rescue one patient who took high doses of digitalis severely poisoned to be successful. Since then, many patients with digitalis poisoning have been treated with this antibody fragment. Experimental methods and results I. In vitro incubation of detoxification Healthy mice, weighing 24 ± 2g, measured intraperitoneal injection