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将来自川东、南稻区代表性的38个县(市)的水稻纹枯病标样29 6份,按不同的品种、海拔、土质、前作和症状归类,选代表性的标样分离得到10 8个丝核菌菌株。按HCL-Giem sa染色程序和菌丝融合测定法,将108个菌株分为3个菌系:Rhizoctonia solani AG-1和AG-4,以及双核丝核菌的AG-Bb。其比例分别为97%、1%和2%。经致病性测定表明,该各菌系对水稻的致病性有显著差异:R.solani AG-1的大多数菌株最强,双核丝核菌AG-Bb最弱,R.solani AG一4居中。对上述各菌系的培养性状、非特异性酯酶和过氧化物酶同工酶谱进行比较研究发现,不同菌系间在上述诸方面均存在明显差异,而同菌系不同菌株间却具一致性。由此说明,按菌丝融合与否区分丝核菌种群较之现行的其它分类法更能反映其遗传本质和亲缘关系。
A total of 296 samples of rice sheath blight from 38 representative counties (cities) in eastern Sichuan and southern rice areas were selected according to different varieties, altitudes, soil types, prematures and symptoms, and representative standard samples were separated Obtain 108 strains of Rhizoctonia. 108 strains were divided into three strains: Rhizoctonia solani AG-1 and AG-4, and AG-Bb of Rhizoctonia solani by HCL-Giem sa staining procedure and mycelial fusion assay. The proportions are 97%, 1% and 2% respectively. Pathogenicity tests showed that the pathogenicity of each strain of rice was significantly different: most strains of R.solani AG-1 were the strongest, while R. binularis AG-Bb was the weakest, R.solani AG-4 Center. Comparison of the traits, nonspecific esterase and peroxidase isoenzyme patterns of the above strains showed that there were significant differences among the different strains in the above aspects, but the same among strains of the same strain Sex. This shows that, according to the mycelium fusion or not to distinguish Rhizoctonia populations compared with the current other taxonomies to better reflect the genetic nature and genetic relationship.