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本实验目的探讨具有相同碳原子长度的亚麻酸、亚油酸及油酸处理ＢＥＬ－７４０２人肝癌细胞后对ＤＮＡ合成和甲胎蛋白（ＡＦＰ）分泌抑制的影响。放射免疫法检测肝癌细胞分泌甲胎蛋白（ＡＦＰ）含量，３Ｈ－ＴｄＲ掺入法测定ＤＮＡ合成放射活性（ＣＰＭ）。实验结果表明，三种脂肪酸能抑制肝癌细胞ＤＮＡ合成，其中亚麻酸、亚油酸抑制作用明显，有显著性差异（Ｐ＜０．０１），油酸作用较弱（Ｐ＞０．０５）；４０－５０μｇ／ｍｌ亚麻酸能明显地抑制肝癌细胞ＡＦＰ分泌（Ｐ＜０．０５），亚油酸和油酸与对照相比无显著性差异（Ｐ＞０．０５）。结果显示，三种脂肪酸分子中含有数量不同的双键，脂肪酸对肝癌细胞ＤＮＡ合成和甲胎蛋白分泌的抑制作用存在构效关系，与分子中双键数量有关。本研究扩充了脂肪酸的用途，为癌症病人的保健和治疗提供实验依据
The objective of this experiment was to investigate the effect of linoleic acid, linoleic acid, and oleic acid of the same carbon length on DNA synthesis and AFP secretion inhibition in human hepatoma cells treated with BEL-7402. Radioimmunoassay was used to detect the level of alpha-fetoprotein (AFP) secreted by hepatocellular carcinoma cells. 3H-TdR incorporation was used to determine DNA synthesis radioactivity (CPM). The experimental results show that the three fatty acids can inhibit the DNA synthesis of hepatocellular carcinoma cells, and the linolenic acid and linoleic acid inhibiting effects are significant (P<0.01), and the oleic acid effect is weak (P>0.05). 40-50μg/ml linolenic acid significantly inhibited AFP secretion in hepatoma cells (P<0.05), and linoleic acid and oleic acid had no significant difference compared with the control (P>0.05). The results showed that the three fatty acid molecules contained a different number of double bonds, and the fatty acid had a structure-activity relationship with the inhibition of DNA synthesis and alpha-fetoprotein secretion of hepatoma cells, and was related to the number of double bonds in the molecule. This study expands the use of fatty acids to provide experimental evidence for the care and treatment of cancer patients.