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目的探讨吡格列酮对游离脂肪酸(FFA)作用不同时间介导的β细胞胰岛素分泌异常的干预效果。方法βTC3细胞分为对照组(Con组)、FFA干预组(FFA组)和FFA加吡格列酮共同干预组(FFA+Pio组),分别干预1、72h,放射免疫法检测不同糖浓度刺激的胰岛素分泌,巢式RT-PCR检测FFA受体1(FFA1R)mRNA的表达。结果干预1h,与Con组比较,FFA组低、中、高糖刺激的胰岛素分泌升高(分别为23.8±1.6 vs 20.0±2.0,28.7±1.4 vs 24.5±1.5,51.0±3.0 vs 42.6±3.4,P<0.05),FFA+Pio组与Con组相比无统计学差异,三组FFA1R表达均无差异。干预72h,FFA组低、中、高糖刺激的胰岛素分泌(分别为20.0±2.0,24.5±1.5,42.6±3.4)和FFA1R表达均较Con组低(P<0.05),FFA+Pio组的胰岛素分泌和FFA1R表达介于FFA组与Con组之间。结论吡格列酮对FFA作用不同时间介导的胰岛素分泌损害具有双向调节作用,其长时间作用可能与调节FFA1R表达有关。
Objective To investigate the effect of pioglitazone on the abnormal insulin secretion induced by free fatty acid (FFA) in different time. Methods The βTC3 cells were divided into control group (Con group), FFA group (FFA group) and FFA plus pioglitazone combined intervention group (FFA + Pio group) for 1, 72 hours respectively. Radioimmunoassay was used to detect insulin secretion stimulated by different glucose concentrations , Nested RT-PCR detection of FFA receptor 1 (FFA1R) mRNA expression. Results Compared with Con group, the insulin secretion of low, medium and high glucose stimulated in FFA group was significantly higher than that of Con group (23.8 ± 1.6 vs 20.0 ± 2.0, 28.7 ± 1.4 vs 24.5 ± 1.5, 51.0 ± 3.0 vs 42.6 ± 3.4, P <0.05). There was no significant difference between FFA + Pio group and Con group, but there was no difference between the three groups in FFA1R expression. After intervention for 72h, the insulin secretion (20.0 ± 2.0,24.5 ± 1.5,42.6 ± 3.4, respectively) and FFA1R expression in FFA group were lower than those in Con group (P <0.05) Secretion and FFA1R expression was intermediate between FFA group and Con group. Conclusion Pioglitazone has a bidirectional effect on the secretion of insulin induced by FFA at different times, and its long-term effect may be related to the regulation of FFA1R expression.