以聚甲基丙烯酸环氧丙酯微球 ( PGMA)为基质 ,利用还原氨化方法将肝素键合在其表面上 ,得到一种以肝素为配基的高效亲和色谱填料 ,该填料对抗凝血酶 的亲和容量为 1 .2 mg/g,对抗凝血酶 和人血清白蛋白的回收率分别为 96.9%和 94 .4 % .利用前沿色谱法测定了肝素与抗凝血酶 之间的表观解离常数为 1 .96× 1 0 - 5mol/L.利用该填料可直接从人血浆中分离出抗凝血酶 Using polyglycolmethacrylate microspheres (PGMA) as substrate, heparin was bonded to its surface by reductive amination to obtain a heparin-based high affinity affinity chromatography packing, which was used for anticoagulant The affinity of the enzyme was 1.2 mg / g, and the recoveries of antithrombin and human serum albumin were 96.9% and 94.4%, respectively. The chromatograms of heparin and antithrombin View dissociation constant of 1.96 × 10 ~ 5mol / L. The use of the filler can be directly isolated from human plasma antithrombin