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目的观察驱铅扶正方含药血清对具有神经特性的PC-12细胞的影响。方法制备驱铅扶正方含药血清,运用MTT法首先检测不同浓度醋酸铅对PC-12细胞的影响,确定醋酸铅浓度后检测不同浓度、不同采血时间的驱铅扶正方含药血清对染铅PC-12细胞的影响。结果采血时间为2 h、浓度为10%含药血清能明显提高以浓度为100μmol/L的醋酸铅100μL刺激24 h条件下铅中毒PC-12细胞的生存率。结论驱铅扶正方含药血清对经浓度为100μmol/L的醋酸铅100μL刺激24 h后的PC-12细胞生存率具有提高作用。
Objective To observe the effect of serums of removing lead and reinforcing square on PC-12 cells with neural characteristics. Methods The serum containing lead and Fufangzhengfang was prepared. MTT assay was used to detect the effect of different concentrations of lead acetate on PC-12 cells. After the concentration of lead acetate was determined, different concentrations of lead- 12 cells. Results The blood sampling time was 2 h and the concentration of 10% serum could significantly increase the survival rate of lead poisoning PC-12 cells induced by 100 μL of lead acetate (100 μmol / L) for 24 h. Conclusion The drug-containing serum of Zuadaozhuzhengfang can improve the survival rate of PC-12 cells induced by 100μL of lead acetate (100μmol / L) for 24 h.