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目的 :研究丹参对大鼠肝脏再灌注损伤的保护作用。方法 :采用大鼠肝脏离体非循环灌注模型 ,观察乳酸林格液 (LR液 )中加入丹参后保存大鼠肝脏 1 2h的效果。结果 :实验组ATP含量 (6 .1 5±0 .6 5 ) μmol/g、TAN含量 (1 5 .86± 1 .75 ) μmol/g和AEC含量 (0 .5 8± 0 .0 6 ) μmol/g,明显高于对照组ATP含量 (2 .6 2± 0 .3 4 ) μmol/g、TAN含量 (1 2 .1 6± 1 .86 ) μmol/g和AEC含量 (0 .3 4± 0 .0 3 ) μmol/g ;实验组DHBA含量 (0 .1 5 4± 0 .0 1 4)nmol/g及 [Ca2 +]i荧光值 (4.2 1± 0 .6 0 )明显低于对照组DHBA含量 (0 .2 5 2± 0 .0 1 8)nmol/g及 [Ca2 +]i荧光值 (4.93± 1 .0 3 ) ,差异有显著性 (P <0 .0 5 )。结论 :丹参可明显提高对肝缺血—再灌注损伤的防护作用。其作用机制可能主要与改善肝脏的能量代谢、抗氧自由基作用及钙拮抗作用有关。
Objective: To study the protective effect of salvia miltiorrhiza on liver reperfusion injury in rats. METHODS: Rat hepatic isolated acyclic perfusion model was used to observe the effect of adding Salvia miltiorrhiza in lactated Ringer’s solution (LR fluid) on rat liver for 12 hours. Results: In the experimental group, the ATP content (6.15±0.65) μmol/g, the TAN content (1 5 .86± 1.75) μmol/g and the AEC content (0.58±0.06) Μmol/g, significantly higher than the control group ATP content (2.62 ± 0.34) μmol/g, TAN content (1 2. 16 ± 1.86) μmol/g and AEC content (0.34 ± 0 .0 3) μmol/g; DHBA content (0.14 5 4 ± 0.014) nmol/g and [Ca 2 +] i fluorescence value (4.2 1 ± 0.60) were significantly lower in the experimental group than in the experimental group. In the control group, the DHBA content (0.252 ± 0.018) nmol/g and the [Ca2 +] i fluorescence value (4.93 ± 1.03) were significantly different (P < 0.05). Conclusion: Salvia miltiorrhiza can significantly improve the protective effect of hepatic ischemia-reperfusion injury. Its mechanism of action may be mainly related to improving liver energy metabolism, antioxidant free radicals, and calcium antagonistic effects.