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目的:研究人喉鳞状细胞癌及其癌旁组织中CTGF和OPN表达水平并探讨两者的相互关系及其临床病理意义。方法:41例喉鳞状细胞癌和20例癌旁组织手术切除标本常规制作石蜡包埋切片,CTGF和OPN染色方法为SP免疫组织化学法。结果:41例鳞状细胞癌CTGF和OPN表达阳性率均为25例(61.0%),其评分值分别为2.41±1.60和3.10±1.63,20例癌旁组织CTGF阳性18例(90%)和OPN阳性3例(15.0%),其评分值分别为4.24±1.42和1.12±0.84,癌组织CGTF阳性率及评分明显低于癌旁组织(χ2=5.44,P<0.05;t=4.36,P<0.01),但OPN则相反(χ2=11.44,P<0.01,t=5.08,P<0.01);颈淋巴结未转移和临床分型T1病例CTGF表达阳性率及其评分明显高于颈淋巴结转移和临床分型T3病例(P<0.01);颈淋巴结转移、临床分型T1及组织学分级Ⅰ级病例OPN表达阳性率及其评分明显低于颈淋巴结转移、临床分型T3及组织学分级Ⅲ级病例(P<0.01);CTGF表达评分与OPN表达评分呈密切负相关(r=-0.56,P<0.01)。结论:CTGF和OPN可能是反映喉鳞状细胞癌进展、生物学行为、转移发生及预后的重要生物学标记物。
OBJECTIVE: To study the expression of CTGF and OPN in human laryngeal squamous cell carcinoma and its adjacent tissues and to explore their correlation and clinicopathological significance. Methods: Paraffin-embedded sections were obtained from 41 cases of laryngeal squamous cell carcinoma and 20 cases of para-cancerous tissues. SP immunohistochemistry was used to detect the expression of CTGF and OPN. Results: The positive rates of CTGF and OPN in 41 cases of squamous cell carcinoma were 25 cases (61.0%), the scores were 2.41 ± 1.60 and 3.10 ± 1.63, respectively, and 18 cases (90%) were CTGF and 20 cases of paracancerous tissues The positive rate and score of CGTF in cancer tissues were significantly lower than that in adjacent non-cancerous tissues (χ2 = 5.44, P <0.05; t = 4.36, P < 0.01), but OPN was the opposite (χ2 = 11.44, P <0.01, t = 5.08, P <0.01). The positive rate and score of CTGF in cervical lymph node without metastasis and clinical T1 were significantly higher than those with cervical lymph node metastasis and clinical The positive rates and scores of OPN expression in cervical lymph node metastasis, clinical type T1 and histological grade Ⅰ cases were significantly lower than those in cervical lymph node metastasis, clinical type T3 and histological grade Ⅲ grade (P0.01) (P <0.01). CTGF expression score was negatively correlated with OPN expression score (r = -0.56, P <0.01). Conclusion: CTGF and OPN may be important biological markers to reflect the progress, biological behavior, metastasis and prognosis of laryngeal squamous cell carcinoma.