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目的研究S100B基因rs9722位点基因多态性与肠道病毒71型(EV71)感染手足口病(HFMD)的相关性。方法以124例EV71感染HFMD患儿为研究对象,56例健康儿童作为对照组,两组均进行S100B基因rs9722位点基因多态性检测;并且74例HFMD患儿进行血清S100B蛋白检测。结果 S100B基因rs9722位点检出3种基因型:CC型、CT型和TT型,基因型频率分布符合Hardy-Weinberg平衡。与对照组比较,HFMD组患儿S100B基因rs9722位点TT基因型频率和T等位基因频率明显增高(P<0.01)。重症EV71感染相关HFMD患儿TT基因型频率和T等位基因频率均明显高于普通型患儿(P<0.05)。与痊愈组比较,HFMD不良预后组的S100B基因rs9722位点TT基因型频率和T等位基因频率明显增高(P<0.05)。74例HFMD患儿各基因型的血清S100B蛋白水平以TT基因型最高,CC型最低(P<0.01)。结论 S100B基因rs9722位点T等位基因可能是EV71感染HFMD发展为重症的危险因素。
Objective To investigate the association between rs9722 polymorphism of S100B gene and HFMD in enterovirus 71 (EV71). Methods A total of 124 EV71 children with HFMD were enrolled in this study. 56 healthy children served as control group. The rs9722 polymorphism of S100B gene was detected in both groups. Serum S100B protein was detected in 74 children with HFMD. Results Three genotypes of rs9722 in S100B gene were detected: type CC, type CT and type TT. The frequency distribution of genotypes conformed to Hardy-Weinberg equilibrium. Compared with the control group, TT genotype frequency and T allele frequency of S100B gene rs9722 in HFMD group were significantly increased (P <0.01). TT genotype frequency and T allele frequency were significantly higher in HFMD children with severe EV71 infection than those in normal children (P <0.05). Compared with the recovery group, TT genotype frequency and T allele frequency of S100B rs9722 in HFMD poor prognosis group were significantly higher (P <0.05). The genotypes of 74 HFMD patients had the highest TT genotype and lowest CC genotype (P <0.01). Conclusion The T allele of rs9722 in S100B gene may be a risk factor for developing severe HFMD in EV71.