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目的:建立愈合王中马齿苋的鉴别方法和4种维生素的含量测定方法。方法:采用薄层色谱法鉴别马齿苋,采用高效液相色谱法测定维生素的的含量,色谱柱为Agilent XDB C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.05%庚烷磺酸钠(磷酸调p H到3.0)(15∶85),检测波长为273 nm,流速:1.0 ml·min~(-1),柱温:35℃,进样量:20μl。结果:薄层鉴别斑点清晰,分离度良好,且阴性对照无干扰。维生素C在80.60~403.00μg·ml~(-1)范围内,烟酰胺在20.10~100.50μg·ml~(-1)范围内,维生素B6在20.36~101.80μg·ml~(-1)范围内,维生素B2在12.24~61.20μg·ml~(-1)范围内,与峰面积呈良好的线性关系。维生素C、烟酰胺、维生素B6和维生素B2回收率分别为99.34%,99.64%,99.29%和99.09%,RSD分别为0.98%,0.20%,1.23%和1.48%。结论:所建方法简便、准确,重复性好,可用于愈合王的质量控制。
Objective: To establish a method for the determination of purslane and the determination of four vitamins in Wang. Methods: The purslane was identified by TLC. The content of vitamins was determined by HPLC. The column was Agilent XDB C 18 column (250 mm × 4.6 mm, 5 μm) with mobile phase of acetonitrile -0.05 % Sodium heptanesulfonate (pH adjusted to pH 3.0) (15:85), detection wavelength at 273 nm, flow rate: 1.0 ml · min -1, column temperature: 35 ℃, injection volume: 20μl. Results: TLC identification spots clear, good resolution, and negative control without interference. Vitamin C was in the range of 80.60 ~ 403.00μg · ml ~ (-1), niacinamide was in the range of 20.10 ~ 100.50μg · ml ~ (-1), and vitamin B6 was in the range of 20.36 ~ 101.80μg · ml ~ (-1) , Vitamin B2 in the range of 12.24 ~ 61.20μg · ml ~ (-1), and the peak area showed a good linear relationship. The recoveries of vitamin C, nicotinamide, vitamin B6 and vitamin B2 were 99.34%, 99.64%, 99.29% and 99.09%, respectively. The RSDs were 0.98%, 0.20%, 1.23% and 1.48%, respectively. Conclusion: The method is simple, accurate and reproducible. It can be used for the quality control of healing king.