观察水稻叶片的气孔数,一般采用“火棉胶印迹法”或“染色固定法”;其效率甚低,形状易模糊不清;尤其是印迹不全或染色固定欠佳时,会影响其准确度。 我们根据气孔的开闭对温湿度的要求,用低倍显微镜直接观察气孔,并对气孔的数目(密度)用传统印迹法和直接观察法进行了对比,取得了较好的效果(表1) Observed the number of stomatal holes in rice leaves, the general use of “cotton wool blotting method” or “stained fixation method”; its efficiency is very low, the shape is easy to vague; especially imprinting incomplete or staining poorly fixed, it will affect its accuracy . According to the opening and closing of the temperature and humidity requirements for stomatal opening, with a low magnification microscope directly observed stomata, and the number of stomata (density) compared with the traditional blot method and direct observation, and achieved good results (Table 1)