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本实验比较观察了新鲜分离和经体外培养36小时的人外周血树突状细胞DC-0和DC-36,在GM-CSF和TNF作用下,对淋巴因子和PHA激活的杀伤细胞LPAK体外杀伤人肝癌细胞林BEL-7402的影响.实验分为DC-O和DC-3两大组,每一大组又分为LPAK+DC(DC-O或DC-36,下同)组,LPAK+DC+GM-CSF(500u/ml,100u/ml)组和LPAK+DC+TNF(5000u/ml,500,u/ml)组,各组均采用LPAK:BEL-7420为5:1和10:1两种效靶比,培养48小时后用中性红摄人比色法检测LPAK细胞的细胞毒活性.结果显示,加入GM-CSF各组的细胞毒活性明显增高(P<0.01),在浓度为100u/ml时已达到最大效应,提高浓度并无意义(P>0.05).加入TNF各组的细胞毒活性也明显提高(P<0.01),但活性随剂量增大而增强(P<
This experiment compared the freshly isolated and in vitro cultured human peripheral blood dendritic cells DC-0 and DC-36 in vitro for 36 hours. Under the action of GM-CSF and TNF, in vitro killing of lymphokines and PHA-activated killer cells LPAK. Effects of Human Hepatocellular Carcinoma Cell Line BEL-7402. The experiments were divided into two groups: DC-O and DC-3. Each large group was divided into LPAK+DC (DC-O or DC-36, the same below) group. LPAK+ DC+GM-CSF (500u/ml, 100u/ml) group and LPAK+DC+TNF (5000u/ml, 500, u/ml) group, each group used LPAK:BEL-7420 5:1 and 10: 1The effect of target ratio, after 48 hours culture, the cytotoxic activity of LPAK cells was detected by neutral red colorimetric assay. The results showed that the cytotoxic activity of GM-CSF was significantly increased in each group (P<0.01). At the concentration of 100u/ml, the maximum effect was achieved, and there was no significance in increasing the concentration (P>0.05). The cytotoxic activity of each group added with TNF also increased significantly (P<0.01), but the activity increased with increasing dose (P<