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探讨流式细胞术检测人巨细胞病毒活动性感染检测方法的可行性及效果评价。分离人外周血白细胞,以商品化的小鼠抗人巨细胞病毒pp65抗原单克隆抗体为一抗,FITC标记的羊抗小鼠IgG抗体为二抗,采用流式细胞术对外周血人巨细胞病毒pp65抗原进行检测。同时采用间接免疫荧光法对外周血人巨细胞病毒pp65抗原进行检测。采用配对χ2检验对两种方法的检测效果进行评价。临床送检的65份疑似为人巨细胞病毒感染病人外周血标本中,间接免疫荧光法检出阳性9份,流式细胞术检出阳性11份,两种方法阳性检出率差异无统计学意义(P>0.05)。采用流式细胞术可定量检测外周血人巨细胞病毒pp65抗原,与间接免疫荧光法检测结果无统计学差异,可在临床推广使用。
To investigate the feasibility and effect evaluation of flow cytometry in the detection of human cytomegalovirus active infection. Peripheral blood leucocytes were isolated and the commercial mouse anti-human cytomegalovirus pp65 antigen monoclonal antibody was used as the primary antibody. The FITC-labeled goat anti-mouse IgG antibody was used as the secondary antibody. Flow cytometry was performed on peripheral blood human giant cells Virus pp65 antigen test. At the same time, indirect immunofluorescence was used to detect the pp65 antigen of human peripheral blood cytomegalovirus. The paired χ2 test was used to evaluate the efficacy of the two methods. In the clinical samples of 65 suspected human cytomegalovirus infected patients, 9 were positive by indirect immunofluorescence assay and 11 were positive by flow cytometry. There was no significant difference between the two methods (P> 0.05). Flow Cytometry can detect peripheral blood cytomegalovirus pp65 antigen, and indirect immunofluorescence test results showed no significant difference, can be used in clinical promotion.