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目的探讨电刺激对缺氧缺血性脑损伤新生大鼠脑组织碱性成纤维细胞生长因子(b FGF)的影响。方法选取60只7日龄清洁级SD雄性大鼠随机分为电刺激组、模型组以及假手术组,制作大鼠缺氧缺血性脑损伤模型。电刺激组为大鼠术后12h进行电刺激治疗,模型组和假手术组不给予刺激。实验结束后各取5只大鼠处死,以免疫组化技术检测b FGF阳性细胞数,以RT-PCR检测b FGF基因水平表达。结果电刺激组、模型组和假手术组第3、7、14、21d在海马齿状回区Brd U阳性细胞数分别为(24.50±5.07、53.13±5.44、47.88±5.25、19.75±4.53)、(13.00±3.74、23.75±4.17、19.00±3.93、14.23±3.92)、(13.25±3.84、18.50±3.59、20.25±4.40、14.25±3.81),模型组和假手术组不同时间段Brd U阳性细胞数差异无统计学意义(P>0.05),电刺激组高于模型组和假手术组(P<0.05)。电刺激组、模型组和假手术组第3、7、14、21d大鼠b FGF m RNA分别为(0.36±0.12、0.75±0.05、0.72±0.13、0.35±0.10)、(0.24±0.04、0.41±0.07、0.32±0.06、0.25±0.05)、(0.24±0.06、0.25±0.05、0.28±0.04、0.25±0.04),电刺激组大鼠不同时间段b FGF m RNA表达显著高于模型组和假手术组(P<0.05),模型组和假手术组差异无统计学意义(P>0.05)。结论缺氧缺血性脑损伤新生大鼠通过电刺激后脑组织b FGF阳性细胞数目增多,能够加快其脑部神经元修复。
Objective To investigate the effect of electrical stimulation on basic fibroblast growth factor (b FGF) in neonatal rats with hypoxic-ischemic brain damage. Methods Sixty male Sprague-Dawley (SD), 7-day-old male SD rats were randomly divided into electrical stimulation group, model group and sham operation group to make a rat model of hypoxic-ischemic brain damage. The rats in the electrical stimulation group were treated with electrical stimulation 12h after operation, while those in the model group and sham operation group were not stimulated. Five rats were sacrificed after the experiment. The number of b FGF positive cells was detected by immunohistochemistry and the expression of b FGF gene was detected by RT-PCR. Results The numbers of BrdU positive cells in the hippocampal dentate gyrus of the 3, 7, 14, 21 d groups were (24.50 ± 5.07, 53.13 ± 5.44, 47.88 ± 5.25 and 19.75 ± 4.53, (13.00 ± 3.74,23.75 ± 4.17,19.00 ± 3.93,14.23 ± 3.92), (13.25 ± 3.84,18.50 ± 3.59,20.25 ± 4.40,14.25 ± 3.81). The numbers of Brd U positive cells in model group and sham operation group at different time points The difference was not statistically significant (P> 0.05). The electrical stimulation group was higher than the model group and the sham operation group (P <0.05). The levels of bFGF m RNA in electrical stimulation group, model group and sham operation group were (0.36 ± 0.12,0.75 ± 0.05,0.72 ± 0.13,0.35 ± 0.10), (0.24 ± 0.04,0.41 ± 0.07,0.32 ± 0.06,0.25 ± 0.05), (0.24 ± 0.06,0.25 ± 0.05,0.28 ± 0.04,0.25 ± 0.04). The expression of b FGFmRNA in electrical stimulation group was significantly higher than that in model group There was no significant difference between the model group and the sham operation group (P> 0.05). Conclusion The number of bFGF positive cells in brain tissue of neonatal rats with hypoxic-ischemic brain damage increased after electrical stimulation, which can accelerate the neuronal repair of the brain.