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目的探讨低氧分压对大鼠勃起功能障碍(ED)的影响。方法48只成年白色雄性SD大鼠随机分为对照组和实验组,每组按照实验时间(2周、6周、10周)分为3个亚组,每个亚组8只。实验组置于密闭低氧舱中饲养,对照组在正常环境中饲养,其他条件相同。分别观察其勃起功能,采用免疫组化SP法检测神经源性一氧化氮合成酶(nNOS)阳性神经纤维的数量、内皮源性一氧化氮合成酶(eNOS)的表达。结果实验组与对照组比较:(1)大鼠勃起次数明显降低(P<0.001);(2)nNOS阳性神经纤维数量、eNOS蛋白的表达均有显著性差异(P<0.01)。实验组间比较:勃起次数6周组明显低于2周组,10周组稍高于6周组;nNOS阳性神经纤维数量有显著性差异(P<0.01);eNOS的表达6周组最低,10周组有所回升。结论低氧分压致大鼠勃起功能受损和nNOS、eNOS的表达下降。nNOS染色阳性神经纤维数量的减少、eNOS表达的下降,可能是低氧分压环境中大鼠ED发生的原因之一。
Objective To investigate the effect of hypoxic partial pressure on erectile dysfunction (ED) in rats. Methods 48 adult white male Sprague-Dawley rats were randomly divided into control group and experimental group. Each group was divided into 3 subgroups according to the experimental time (2 weeks, 6 weeks and 10 weeks), and each subgroup was divided into 8 subgroups. The experimental group was housed in a closed hypoxic chamber, and the control group was housed in normal environment with other conditions being the same. The erectile function was observed respectively. The number of nerve-derived nitric oxide synthase (nNOS) positive nerve fibers and the expression of endothelial nitric oxide synthase (eNOS) were detected by immunohistochemical SP method. Results Compared with the control group, the number of erectile nerve in experimental group was significantly decreased (P <0.001). (2) The number of nNOS positive nerve fibers and eNOS protein expression were significantly different (P <0.01). The number of erectile nerve in 6 weeks group was significantly lower than that in 2 weeks group, 10 weeks group was slightly higher than 6 weeks group; the number of nNOS positive nerve fibers was significantly different (P <0.01); eNOS expression was the lowest in 6 weeks group, 10 weeks group has picked up. Conclusion Hypoxic partial pressure induced impaired erectile function and decreased expression of nNOS and eNOS in rats. The decrease of nNOS-positive nerve fibers and the decrease of eNOS expression may be one of the reasons of rat ED in hypoxic partial pressure environment.