论文部分内容阅读
目的 了解严重急性呼吸综合征(severeacuterespiratorysyndrome ,SARS)患儿SARS相关冠状病毒(SARS CoV)特异性抗体水平和与SARS患儿密切接触的成人有无隐性感染。方法 采用间接免疫荧光(IFA)和ELISA两种方法检测北京市2 4例恢复期儿童SARS患者和2 6名与其密切接触的家长血清SARS CoV特异抗体,其中IFA法检测IgM和IgG抗体,ELISA检测IgM和混合抗体;同时通过入户问卷收集流行病学资料。结果 ( 1 )IFA法测定血清SARS CoV IgG的阳性者1 0例( 4 2 % ) ,SARS CoV IgM阳性2例( 8% ) ;经ELISA法测定SARS CoV混合抗体的阳性者9例( 3 8% )。( 2 )流行病学资料显示抗体阳性的1 0例患儿中有明确SARS接触史的患儿8例,而在抗体阴性的1 3例中仅1例阳性(P <0 . 0 5)。( 3 ) 1位受检家长(患儿的祖母)IFA法检测SARS CoV IgG和ELISA法检测混合抗体均为阳性,约占4% ( 1 / 2 6) ,该患儿的祖父亦为SARS患者。结论 ( 1 )有SARS接触史的患儿在抗体阳性组的比例较之在抗体阴性组的比例明显增高,提示流行病学史在儿科SARS临床诊断中具有重要价值。( 2 )未能证实与儿童SARS患者接触的成人中存在隐性感染。
Objective To investigate the level of SARS-CoV-specific antibodies in children with severe acute respiratory syndrome (SARS) and the presence of latent infection in adults with close contact with children with SARS. Methods Two methods of indirect immunofluorescence (IFA) and ELISA were used to detect serum SARS CoV specific antibodies in 24 cases of convalescent childhood SARS and 26 parents in Beijing. IFA was used to detect IgM and IgG antibodies. ELISA and ELISA IgM and mixed antibodies; epidemiological data were also collected from household questionnaires. Results (1) Serum SARS CoV IgG was detected by IFA in 10 cases (42%) and 2 cases (8%) of SARS CoV IgM positive cases. ELISA positive cases of SARS CoV mixed antibody in 9 cases (38 %). (2) Epidemiological data showed that there were 8 out of 10 cases of antibody-positive children with definite history of SARS exposure, while only 1 case was positive in antibody-negative 13 cases (P <0.05). (3) A total of 4% (1/2 6) of the mixed antibodies were detected by the IFA method for detecting SARS CoV IgG and ELISA. The grandfather was also a SARS patient . Conclusions (1) The proportion of patients with history of SARS exposure in antibody-positive group is significantly higher than that in antibody-negative group, suggesting that epidemiological history is of great value in the clinical diagnosis of pediatric SARS. (2) There is no evidence of a latent infection in adults who have contact with children with SARS.