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目的 建立糖尿病性视网膜并发症 (DiabeticRetinopathy ,DR)动物模型。方法 本研究选用 4只健康成熟的雄性恒河猴分别以剂量为 6 0mg kg、4 5mg kg静脉一次注射及 30mg kg静脉二次注射 (中间间隔 15d)链脲佐菌素 (Streptozotocin ,STZ)。结果 使用不同剂量的STZ注射恒河猴 ,分别造成了胰岛素依赖性糖尿病 (InsulinDepen dentDiabetesMellitus ,IDDM)及慢性持续性高血糖症 (StateofChronicHyperglycemia,SCH)两种类型的模型 ,从而诱导出不同程度的DR。眼底荧光造影结果显示∶用药后 6 3～ 91d4只实验猴均出现不同程度的视网膜病 ,分别显示早期眼底微血管动脉扩张 ,视网膜出血瘤 ,微血管瘤及新生血管、晚期白内障等。结论 本研究所建立的糖尿病性视网膜并发症模型 ,对于进一步研究糖尿病及其并发症的发病机理 ,筛选及开发治疗糖尿病性视网膜病的新药及药物安全性评价将会具有广阔的应用前景
Objective To establish a diabetic retinopathy (Diabetic Retinopathy, DR) animal model. Methods In this study, 4 healthy male Rhesus macaques were injected intravenously with a dose of 60 mg and 45 mg respectively, and intravenous injection of 30 mg kg of streptozotocin (STZ) intravenously at a median interval of 15 days. Results Rhesus monkeys were injected with different doses of STZ, resulting in two types of insulin-dependent diabetes mellitus (IDDM) and chronic persistent hyperglycemia (SCH), respectively, which induced different degrees of DR. Fundus fluorescein angiography showed that: after 6 to 91 days of treatment, all experimental monkeys showed different degrees of retinopathy, showing early fundus arteriolar dilatation, retinal hemorrhage, microangioma and neovascularization, advanced cataracts and so on. Conclusion The diabetic retinopathy model established in this study will have broad application prospects for the further study on the pathogenesis of diabetes and its complications, screening and developing new drugs and drug safety evaluation for the treatment of diabetic retinopathy