由于山楂种子常规育苗需时较长从而影响了苗木的快速繁殖,为此,进行了山楂离体培养快速繁殖研究,简结如下。材料和方法以大金星品种为试材,在春季取其嫩梢,去掉幼叶,在自来水中冲洗2小时,再用75％的酒精消毒10秒钟、0.1％升汞(加2滴吐温)消毒5分钟,然后用无菌水冲洗4～6次。剪成单芽茎段,放到诱导分化培养基中。 Hawthorn seed conventional seedling takes a long time and thus affect the rapid propagation of seedlings, for which hawthorn rapid culture in vitro studies, summarized as follows. MATERIALS AND METHODS Take Venus varieties as test materials, take their young shoots in spring, remove the young leaves, rinse in tap water for 2 hours, sterilize with 75% alcohol for 10 seconds, add 0.1% mercuric chloride Disinfect for 5 minutes and then rinse 4 to 6 times with sterile water. Cut into single bud stem segments, into differentiation medium differentiation.