用还原型尼克酰胺腺嘌呤二核苷酸脱氢酶组化方法观察 E14d起至生后 2 8d这段时间大鼠纹状体一氧化氮合酶阳性神经元的发育。结果 :胚龄 16d时 ,尾壳核内已有一氧化氮合酶的表达 ,此时细胞数量少 ,细胞圆形 ,无突起或仅有一短的突起 ;胚龄 18d一氧化氮合酶阳性神经元的数量增多 ;生后 1d一氧化氮合酶阳性细胞胞体增大 ,有些突起呈点线状 ;生后 7d一氧化氮合酶阳性神经元的胞体及突起进一步发育 ,突起呈线状 ,其上出现膨体样结构 ;14d时 ,神经元进一步发育分化 ,突起变多、变长 ,出现分支 ,阳性纤维主要出现在纹状体特有的片状区内 ;2 1d时分支进一步增多 ;到生后 2 8d基本达到成体水平。一氧化氮合酶阳性神经元的分布在胚胎和生后 1d时出现于尾壳核的腹外侧 ,到生后第 7d方分布于整个尾壳核 ,但始终以腹外侧密度最大 ,且尾壳核外侧的细胞在形态上要较内侧成熟早。在苍白球中一氧化氮合酶阳性神经元少见 The reduction of nitric oxide adenine dinucleotide dehydrogenase histochemical method was used to observe the development of nitric oxide synthase (nNOS) -positive neurons in the striatum of rats during the period from E14 to 28 days after birth. Results: The expression of nitric oxide synthase was present in the caudate putamen nucleus 16 days after embryo implantation, when the number of cells was small, the cells were round, no protuberance or only a short protuberance. The expression of nitric oxide synthase positive neurons The number of nitric oxide synthase positive cells increased 1d after birth, and some of the protuberances were dotted. After 7d, the cell bodies and protrusions of nitric oxide synthase positive neurons were further developed, and the protrusions appeared linear There was a bulge-like structure. On the 14th day, neurons further developed and differentiated, with more protrusions, longer branches and branching. Positive fibers mainly appeared in the striatum in the lamina cribrosa. Branches were further increased after 21 days. After birth 28d basically reached the adult level. The distribution of nitric oxide synthase positive neurons appeared on the ventrolateral side of the caudate putamen on embryonic and postnatal day 1 and distributed throughout the caudate putamen on the 7th day after birth, Cells outside the nucleus are morphologically older than inside. Nitric oxide synthase positive neurons are rare in globus pallidum