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目的:研究TNFRSF1B+676位点(rs1061622)基因多态性对湖南汉族人群类风湿关节炎(rheumatoid arthritis,RA)风险的影响。方法:标本来源于112例湖南汉族RA患者及129例健康献血者。TNFRSF1B+676基因多态性采用限制性片段长度多态性(PCR-RFLP)法分析。血清可溶性肿瘤坏死因子受体II(soluble tumor necrosis factor receptor II,sT NFR II)水平检测采用ELISA方法。结果:湖南汉族人群中,RA组TNFRSF1B+676基因型构成与正常对照组比较无明显差异(GG/TG/TT:5/62/45 vs 9/56/64,P=0.167);女性RA患者TNFRSF1B+676基因型构成比与女性正常对照比较差异有统计学意义(GG/TG/TT:3/49/24 vs 8/28/48,P<0.001);RA组TNFRSF1B+676基因位点T或G等位基因频率与正常对照组比较无明显差异(P>0.05)。RA患者血清sT NFR II水平明显高于正常对照组[(7.83±2.61)ng/mL vs(4.32±1.67)ng/mL,P<0.001);无论是RA患者或正常对照,TNFRSF1B+676位点GG,TG,TT三种不同基因型血清s TNFR II水平比较,差异无统计学意义(P>0.05)。结论:在湖南汉族人群中,TNFRSF1B+676基因多态性与RA易感性无明显关联。
Objective: To investigate the effect of TNFRSF1B + 676 (rs1061622) gene polymorphism on the risk of rheumatoid arthritis (RA) in Hunan Han population. Methods: The specimens were from 112 Han patients with RA in Hunan and 129 healthy blood donors. TNFRSF1B + 676 gene polymorphism was analyzed by restriction fragment length polymorphism (PCR-RFLP). Serum soluble tumor necrosis factor receptor II (sT NFR II) levels were measured by ELISA. Results: The genotypes of TNFRSF1B + 676 in RA group were not significantly different from those in normal controls (GG / TG / TT: 5/62/45 vs 9/56/64, P = 0.167) The genotype of TNFRSF1B + 676 was significantly different from the normal controls (GG / TG / TT: 3/49/24 vs 8/28/48, P <0.001). The TNFRSF1B + 676 locus T Or G allele frequency compared with the normal control group no significant difference (P> 0.05). The level of serum sT NFR II in RA patients was significantly higher than that in the normal controls [(7.83 ± 2.61) ng / mL vs (4.32 ± 1.67) ng / mL, P <0.001) There was no significant difference in the levels of serum s TNFR II between GG, TG and TT genotypes (P> 0.05). CONCLUSION: There is no significant association between TNFRSF1B + 676 polymorphism and RA susceptibility in Hunan Han population.