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目的通过11,二苯基-2-苦肼基自由基(DPPH.)法测定红柳醇提物各组分清除自由基的能力。方法将红柳醇提物依次用石油醚、乙酸乙酯、正丁醇进行萃取,各组分都配置成相当于原药材50mg.mL-1的溶液,通过DPPH.法测定各组分的清除自由基的能力,并计算各组分的清除50%自由基时样品浓度(IC50)。结果正丁醇层清除自由基的能力最强I,C50为0.55mg.mL-1,其次是萃后水层(IC50为1.47mg.mL-1),乙酸乙酯层(IC50为3.96mg.mL-1)和石油醚层(IC50为4.78mg.mL-1)。结论红柳醇提物各组分对DPPH.均具有一定清除作用,具有体外抗氧化性,可作为有效的天然自由基清除剂,具有很大的开发利用前景。
OBJECTIVE To determine the free radical scavenging ability of the constituents of the extract of Phyllanthus albus by 11, diphenyl-2-hydrazinyl free radical (DPPH.) Method. Methods Triterpenoids extracts were extracted with petroleum ether, ethyl acetate and n-butanol. The components were all equilibrated with 50 mg.mL-1 of the crude drug, and the components were cleared by DPPH. The capacity of the base was calculated and the sample concentration (IC50) at 50% free radical scavenging for each fraction was calculated. Results The n-butanol layer had the strongest ability to scavenge free radicals. The C50 was 0.55mg.mL-1, followed by the aqueous extract with IC50 of 1.47mg.mL-1 and the ethyl acetate layer with IC50 of 3.96mg. mL-1) and petroleum ether layer (IC50 4.78 mg.mL-1). Conclusion The constituents of Triticum aestivum alcohol extract have certain scavenging effects on DPPH, have antioxidant activity in vitro and can be used as effective natural free radical scavenger, which has great prospect of development and utilization.