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A simple,fast and sensitive capillary electrophoresis(CE) strategy combined with chemiluminescence(CL) detection for analysis of ofloxacin(OF) enantiomers was established in the present work.Sulfonated p-cyclodextrin(β-CD) was used as the chiral additive being added into the running buffer of luminol-diperiodatocuprate(Ⅲ)(K_[Cu(HIO_6)_2],DPC) chemiluminescence system.Under the optimum conditions,the proposed method was successfully applied to separation and analysis of OF enantiomers with the detection limits(S/N=3) of 8.0 nM and 7.0 nM for levofloxacin and dextrofloxacin,respectively.The linear ranges were both 0.010-100 μM.The method was utilized for analyzing OF in urine;the results obtained were satisfactory and recoveries were 89.5-110.8%,which demonstrated the reliability of this method.This approach can also be further extended to analyze different commercial OF medicines.
A simple, fast and sensitive capillary electrophoresis (CE) strategy combined with chemiluminescence (CL) detection for analysis of ofloxacin (OF) enantiomers was established in the present work. Sulfonated p-cyclodextrin (β-CD) was used as the chiral additive being added into the running buffer of luminol-diperiodatocuprate (III) (K_ [Cu (HIO_6) _2], DPC) chemiluminescence system. Undertaking applied to separation and analysis of OF enantiomers with the detection limits S / N = 3) of 8.0 nM and 7.0 nM for levofloxacin and dextrofloxacin, respectively. These method was utilized for analyzing OF in urine; the results obtained were satisfactory and recoveries were 89.5-110.8 %, which demonstrated the reliability of this method. This approach can also be further extended to analyze different commercial OF medicines.