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报道了一种新型恒流式颗粒计数技术及微流控芯片装置。通过在微流控芯片主通道样品孔两端分别插入一定尺寸的Zn和Cu电极,可在主通道内形成较为稳定的恒流源。当绝缘材质的颗粒通过检测区域时,会从检测区域排出等体积的电解质溶液,使检测区域的电阻变大。该电阻变化会使检测区域两端电压发生改变,并以脉冲电压信号的形式被检测系统检测到,从而实现颗粒计数。实验结果表明:当主通道两端电压相同时,恒电流系统的检测信噪比要远远高于直流稳压电源;随着Zn电极面积的增加,相同尺寸颗粒经过检测区产生的电压信号幅值也随之增加。原电池自驱动微流控芯片装置结构简单、检测精度高,无需外接昂贵的恒流源装置,可发展成为便携式仪器,有望用于某些特殊场合的现场检测。
Reported a new type of constant current particle counting technology and microfluidic chip device. By inserting a certain size of Zn and Cu electrodes at both ends of the sample channel of the main channel of the microfluidic chip, a stable constant current source can be formed in the main channel. When particles of insulating material pass through the detection area, an equal volume of electrolyte solution is discharged from the detection area to increase the resistance of the detection area. This resistance change causes the voltage across the detection area to change and is detected by the detection system in the form of a pulse voltage signal to achieve particle counting. The experimental results show that when the voltage across the main channel is the same, the detection signal-to-noise ratio of the constant current system is much higher than that of the DC power supply. As the area of the Zn electrode increases, the amplitude of the voltage signal Also will increase. The original cell self-driven microfluidic chip device has the advantages of simple structure, high detection precision, no need of external expensive constant current source device, which can be developed into a portable instrument and is expected to be used for on-site detection in some special occasions.