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番茄抗病蛋白Tm-2~2与其等位感病蛋白tm-2在序列上相对保守但也有一定的差异性,研究这些差异对于了解Tm-2~2抗病蛋白的结构和功能起着重要作用。本研究构建了不同的Tm-2~2/tm-2置换突变体,在本生烟草上利用农杆菌注射法共表达突变体及其效应因子MP或TMV-GFP载体。通过对植物坏死表型以及病毒量变化的观察,发现当Tm-2~2ARC结构域中RNBS-D保守基序中的第413位甘氨酸突变成tm-2中的丝氨酸后,Tm-2~2介导的对烟草花叶病毒属病毒的抗性减弱。这说明RNBS-D保守基序对于Tm-2~2行使其抗病功能是至关重要的。
The Tm-2 ~ 2 and Tm-2 are relatively conserved but also have some differences in sequence, and it is important to understand these differences in understanding the structure and function of the Tm-2 ~ 2 resistance protein effect. In this study, different Tm-2 ~ 2 / tm-2 substitution mutants were constructed. Agrobacterium-mediated mutagenesis and its effector factor MP or TMV-GFP vector were used to clone tobacco. By observing the phenotype of plant necrosis and the change of virus quantity, it was found that when the 413th glycine in RNBS-D conserved motif in Tm-2 ~ 2ARC domain was mutated to serine in tm-2, Tm-2 ~ 2 mediated resistance to tobacco mosaic virus. This indicates that the RNBS-D conserved motif is crucial for Tm-2 ~ 2 to exercise its disease-fighting function.