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单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)是白色脂肪细胞分泌的炎症趋化刺激因子,属于趋化因子CC亚族,可促进肿瘤血管形成和细胞外基质降解,从而促进肿瘤细胞的浸润与转移。沉默MCP-1基因可显著抑制恶性肿瘤生长及转移,但其作用的分子机制尚不完全清楚。本研究应用小干扰RNA技术沉默人食管癌EC109细胞中MCP-1表达。细胞划痕试验显示,与对照组相比,沉默MCP-1基因可明显抑制食管癌EC109细胞迁移能力。Transwell侵袭实验显示,沉默MCP-1基因后,EC109细胞侵袭能力降低。Western印迹试验和RT-PCR试验揭示,沉默MCP-1基因后,细胞中MMP-7、MMP-9、TGF-β_1及VEGF表达水平显著下降。研究结果提示,沉默MCP-1基因可通过抑制MMP-7、MMP-9、TGF-β_1及VEGF表达,降低癌细胞迁移及侵袭能力。
Monocyte chemoattractant protein-1 (MCP-1), an inflammatory chemotactic stimulator secreted by white adipocytes, belongs to chemokine CC subfamily and promotes tumor angiogenesis and extracellular matrix degradation. Thus promoting the infiltration and metastasis of tumor cells. Silencing MCP-1 gene can significantly inhibit the growth and metastasis of malignant tumors, but the molecular mechanism of its action is not fully understood. In this study, small interfering RNA technology was used to silence the expression of MCP-1 in human esophageal cancer cell line EC109. Cell scratch assay showed that silencing MCP-1 gene significantly inhibited esophageal EC109 cell migration compared with control. Transwell invasion assay showed that the silencing of MCP-1 gene reduced the invasiveness of EC109 cells. Western blot and RT-PCR revealed that the expression of MMP-7, MMP-9, TGF-β 1 and VEGF were significantly decreased after silencing MCP-1 gene. The results suggest that silencing MCP-1 gene can reduce the migration and invasion of cancer cells by inhibiting the expression of MMP-7, MMP-9, TGF-β 1 and VEGF.