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目的探讨去甲斑蝥素(NCTD)对人类胃癌 SGC-7901细胞凋亡相关基因表达的影响。方法实验分对照组、5-Fu 组、NCTD 组和5-Fu+NCTD 组,分别应用 MTT、流式细胞术、免疫组化SABC 法检测 NCTD 对体外培养胃癌 SGC-7901细胞的杀伤抑制率、凋亡率和 survivin、bcl-2、caspase-3的表达。结果 NCTD 对人类胃癌 SGC-7901细胞生长有抑制作用,呈剂量-时间效应关系;凋亡率由(8.30±1.49)%上升到(20.56±1.32)%;survivin 基因表达由(86.57±4.39)%下降到(26.11±2.27)%;bcl-2基因表达由(85.35±3.25)%下降到(30.26±1.83)%;caspase-3基因表达由(54.49±3.07)%上升到(92.78±2.47)%;与5-Fu 联合应用上述作用更明显。结论 NCTD 能抑制人类胃癌 SGC-7901细胞的生长;与5-Fu 具有协同作用,其机制可能与其诱导细胞凋亡,影响凋亡相关基因表达有关。
Objective To investigate the effects of norcantharidin (NCTD) on the expression of apoptosis-related genes in human gastric cancer SGC-7901 cells. Methods The control group, 5-Fu group, NCTD group and 5-Fu + NCTD group were used to detect the cytotoxicity of NCTD on gastric cancer SGC-7901 cells by MTT, flow cytometry and immunohistochemical SABC method respectively. Apoptosis rate and the expression of survivin, bcl-2, caspase-3. Results NCTD inhibited the growth of human gastric cancer cell line SGC-7901 in a time-dose-dependent manner. The apoptosis rate increased from (8.30 ± 1.49)% to (20.56 ± 1.32)%, and the expression of survivin gene increased from (86.57 ± 4.39)% (26.11 ± 2.27)%; the expression of bcl-2 gene decreased from (85.35 ± 3.25)% to (30.26 ± 1.83)%; the expression of caspase-3 gene increased from 54.49 ± 3.07% to 92.78 ± 2.47% ; 5-Fu combined with the above-mentioned effect is more obvious. Conclusions NCTD can inhibit the growth of human gastric cancer cell line SGC-7901 and has a synergistic effect with 5-Fu. The mechanism may be related to the induction of apoptosis and the expression of apoptosis related genes.