研究了发根农杆菌介导下的长春花毛状根的诱导及培养。不同的菌种、外植体部位、培养基和代谢产物对毛状根诱导效果不同，最高诱导率分别为：Ａ４菌２５．３％；下胚轴２４．１％；１／２ＭＳ培养基２３．３％。农杆菌诱导转化时间１５～２０ｄ。完成了长春花毛状根的筛选克隆及培养 The induction and culture of hairy roots of Catharanthus roseus under Agrobacterium rhizogenes was studied. Different strains, explants, media and metabolites have different inducing effects on hairy roots, the highest induction rates are: 25.3% of A4 bacteria; 24.1% of hypocotyls; 1 / 2MS medium 23 .3%. Agrobacterium-induced transformation time 15 ~ 20d. Completed the screening of Catharanthus roseus roots and culture