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目的探讨小鼠脑缺血/再灌注后胶质细胞形态学与数量的变化及其临床意义。方法采用随机方法将32只健康昆明雄性小鼠分为假手术组和模型组,每组16只。制备脑缺血/再灌注损伤模型,应用免疫组织化学方法分别标记星形胶质细胞特异性蛋白胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)与小胶质细胞特异性蛋白离子钙接头蛋白抗体(ionized calcium-binding adaptor molecule 1,Iba-1),观察脑缺血/再灌注小鼠海马区胶质细胞形态及数量的变化。结果脑缺血/再灌注损伤后模型组小鼠海马区锥体细胞排列紊乱,细胞脱失明显,细胞核体积变小,深染,呈核固缩。GFAP与Iba-1阳性细胞在形态上表现为胞体肥大,分支变粗;模型组阳性细胞数分别为(12.56±1.58)个/HP和(9.63±1.50)个/HP,均显著多于假手术组,差异具有统计学意义(P<0.01)。结论小鼠局灶性脑缺血/再灌注损伤后海马区胶质细胞过度活化,可能参与加重脑缺血/再灌注后组织损伤及神经元凋亡。
Objective To investigate the changes of glial morphology and quantity after cerebral ischemia / reperfusion in mice and its clinical significance. Methods 32 healthy Kunming male mice were randomly divided into sham operation group and model group, with 16 rats in each group. The model of cerebral ischemia / reperfusion injury was established. The expression of glial fibrillary acidic protein (glial fibrillary acidic protein, glial fibrillary acidic protein (GFAP) and microglia specific protein calcium ion exchanger protein Antibody (Iba-1) was used to observe the changes of glial morphology and number in hippocampus of cerebral ischemia / reperfusion mice. Results After cerebral ischemia / reperfusion injury, the pyramidal cells in hippocampus of mice in the model group were disordered, the cells were detached obviously, and the nucleus became smaller and darker with nuclear pyknosis. GFAP and Iba-1 positive cells were morphologically characterized by hypertrophy of the cell body and thickening of the branches. The numbers of positive cells in the model group were (12.56 ± 1.58) / HP and (9.63 ± 1.50) / HP, respectively, Group, the difference was statistically significant (P <0.01). Conclusion Overexpression of glial cells in the hippocampus after focal cerebral ischemia / reperfusion injury may play an important role in tissue damage and neuronal apoptosis after focal cerebral ischemia / reperfusion.