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目的:建立以反相高效液相色谱法测定复方丹栀逍遥丸中栀子苷含量的方法。方法:色谱柱为Shim-pack-vp-ODS(150mm×4.6mm,5μm),流动相为乙腈-水(15∶85),流速为1.0mL·min-1,检测波长为240nm。结果:栀子苷的进样量在0.11～0.67μg范围内与峰面积积分值呈良好的线性关系(r=0.9992);平均回收率为96.7%,RSD=1.2%(n=6)。结论:本方法简单、准确、快速,可用于复方丹栀逍遥丸的质量控制。
Objective: To establish a method for the determination of geniposide in Fufang Danzhi Xiaoyao Pills by reversed-phase high performance liquid chromatography. Methods: The chromatographic column was Shim-pack-vp-ODS (150mm × 4.6mm, 5μm). The mobile phase was acetonitrile-water (15:85). The flow rate was 1.0mL · min-1. Results: The injection of geniposide showed a good linear relationship with the peak area integral value (r = 0.9992) in the range of 0.11-0.67 μg; the average recovery was 96.7% and the RSD was 1.2% (n = 6). Conclusion: The method is simple, accurate and rapid and can be used for the quality control of Compound Danzhi Xiaoyao Pills.