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目的观察高体积分数氧(高氧)暴露早产儿血单个核细胞(PBMC)血红素加氧酶-1(HO-1)蛋白及mRNA的表达水平和活性变化。方法分别用反转录聚合酶链反应(RT-PCR)技术、Westernblot方法对60例吸入高氧的早产儿(高氧组)血中PBMC中HO-1mRNA及蛋白表达水平进行检测,并测定血清胆红素水平代表HO-1活性及血浆一氧化碳血红蛋白(COHb)百分比,并与吸入常压空气的早产儿60例对照进行比较。结果与空气组相比,高氧组早产儿PBMC中HO-1mRNA及蛋白表达明显增加[(0.64±0.05)vs(0.13±0.03),(1.78±0.25)vs(1.12±0.31),Pa<0.01)],血清胆红素生成量及血浆COHb水平也显著升高[(76.25±11.33)nmol.L-1.h-1vs(53.47±8.73)nmol.L-1.h-1,(6.73±0.42)μmol.L-1vs(2.47±0.34)μmol.L-1,Pa<0.01]。结论高氧暴露可上调早产儿血PBMC中HO-1mRNA及蛋白质表达水平,推测HO-1可能参与早产儿高氧肺损伤。
Objective To investigate the expression and activity of heme oxygenase-1 (HO-1) protein and mRNA in blood mononuclear cells (PBMC) exposed to high volume fraction of oxygen (hyperoxia) exposed premature infants. Methods The HO-1 mRNA and protein levels in PBMC of 60 infants with hyperoxia (hyperoxia group) were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot respectively. The serum levels of HO- Bilirubin levels represent the activity of HO-1 and the percentage of plasma CO-hemoglobin (COHb) and are compared with 60 controls in preterm infants who inhale atmospheric air. Results Compared with air group, the expression of HO-1 mRNA and protein in PBMC of hyperoxia group was significantly higher than that of air group [(0.64 ± 0.05) vs (0.13 ± 0.03) vs (1.78 ± 0.25) vs (1.12 ± 0.31), Pa ), Serum bilirubin production and plasma COHb levels were also significantly increased [(76.25 ± 11.33) nmol.L-1.h-1 vs (53.47 ± 8.73) nmol.L-1.h-1, (6.73 ± 0.42) μmol.L-1vs (2.47 ± 0.34) μmol.L-1, Pa <0.01]. Conclusion Hyperoxia exposure can up-regulate the expression of HO-1 mRNA and protein in PBMC of preterm infants, suggesting that HO-1 may be involved in hyperoxia-induced lung injury in preterm infants.