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合成了一种新型四(对磺酸基偶氮苯基-4-氨基磺酰基)铝(Ⅲ)氯酞菁(S-AlPc),采用元素分析、IR、1H NMR和ESI-MS对配合物的结构进行了表征.S-AlPc中带负电荷的磺酸基与两亲嵌段共聚物聚乙二醇-聚L-赖氨酸(PEG-b-PLL)带正电荷的PLL嵌段通过静电作用自组装合成负载四(对磺酸基偶氮苯基-4-氨基磺酰基)铝(Ⅲ)氯酞菁聚合物纳米粒子(S-AlPc/PbP),直径约为10～20 nm.采用UV-Vis和稳态及瞬态荧光光谱法比较了S-AlPc和S-AlPc/PbP的光物理性质,并研究了它们对人脐静脉内皮细胞(HUVEC)的摄取量.与S-AlPc相比,包裹S-AlPc的聚合物纳米粒子S-AlPc/PbP的HUVEC摄取量有所提高,且提前1 h达到最大浓度.采用MTT法评价了S-AlPc和S-AlPc/PbP对HUVEC的光动力活性,S-AlPc/PbP的细胞抑制率大于S-AlPc.因此,S-AlPc/PbP是一类很有前景的第三代治疗脉络膜新生血管的光敏剂.
A novel tetrakis (p-sulfonazo phenyl-4-aminosulfonyl) aluminum (Ⅲ) chlorophthalocyanine (S-AlPc) was synthesized and characterized by elemental analysis, IR, 1H NMR and ESI- Structure was characterized by the negatively charged sulfonic acid group of S-AlPc and the amphiphilic block copolymer PEG-b-PLL positively charged PLL block (S-AlPc / PbP) loaded with tetrakis (p-sulfonazo phenyl-4-aminosulfonyl) aluminum (III) chloride with a diameter of about 10 ~ 20 nm by electrostatic interaction. The photophysical properties of S-AlPc and S-AlPc / PbP were compared by UV-Vis and steady-state and transient fluorescence spectroscopy, and their uptake of human umbilical vein endothelial cells (HUVECs) Compared with that of S-AlPc / PbP encapsulated S-AlPc polymer nanoparticles, and reached the maximum concentration 1 h ahead of time.Using MTT method, the effect of S-AlPc and S-AlPc / PbP on HUVEC Therefore, S-AlPc / PbP is a promising third generation photosensitizer for the treatment of choroidal neovascularization.