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目的:观察人参总皂苷对树突状细胞系DC2.4细胞增殖、抗原吞噬和表面分子表达的影响。方法:体外培养DC2.4细胞,加入不同剂量人参总皂苷后,MTT法检测不同时点细胞增殖;流式细胞仪检测细胞表面分子CD40、CD86和MHCⅡ的表达,以及对卵清蛋白抗原的吞噬作用。结果:单纯加入脂多糖(LPS)经培养48h后DC2.4细胞增殖明显(P<0.05),人参总皂苷可明显抑制LPS诱导的DC2.4细胞增殖(P<0.05);此外,人参总皂苷可促进DC2.4细胞吞噬抗原的能力,并协同LPS上调CD40、CD86和MHCⅡ分子表达。结论:人参总皂苷可以维持DC2.4细胞数量的稳态,促进DC2.4细胞吞噬抗原,并与LPS协同促进DC2.4细胞成熟,并具有促进抗原提呈的作用。
Objective: To observe the effects of ginsenosides on proliferation, antigen phagocytosis and surface molecule expression of dendritic cell line DC2.4. Methods: DC2.4 cells were cultured in vitro. After adding different doses of ginsenosides, MTT assay was used to detect the proliferation of cells at different time points. The expression of CD40, CD86 and MHC Ⅱ were detected by flow cytometry and phagocytosis of ovalbumin antigen effect. Results: Proliferation of DC2.4 cells was significantly increased after LPS supplementation for 48 hours (P <0.05), and total ginsenoside could inhibit LPS-induced proliferation of DC2.4 cells (P <0.05). In addition, total ginsenosides Can promote the ability of DC2.4 cells to phagocytize antigen, and cooperate with LPS to up-regulate the expression of CD40, CD86 and MHCⅡ. Conclusion: Ginsenosides can maintain the steady state of the number of DC2.4 cells and promote the phagocytosis of DC2.4 cells, and synergize with LPS to promote the maturation of DC2.4 cells and promote antigen presentation.