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紅松和油松杂交是不易得到种子的。我們試图从研究紅松和油松的大、小袍子发育、受精过程、染色体数目,来寻求其杂交不孕的原因。試驗結果是: 1.紅松由传粉到受精間隔12个月,油松則間隔13个月。二者在授粉后,花粉貯存于珠心頂部的花粉室內,平均溫度約22℃时开始发芽。发芽的花粉在花粉室內越冬,翌春花粉管继续伸长,至6月中旬受精。 2.紅松和油松的染色体数目相差很大,紅松小孢子母細胞內n=6,油松小孢子母細胞內n=12;紅松胚乳細胞內n=6,个别的細胞內也有n=7,8,…,10的,油松的胚乳細胞內則是n=12,也有n=14的。由染色体数目差别之大,也可推知紅松和油松杂交是有困难的。 3.紅松和油松杂交不孕的主要原因是,油松的花粉不能在紅松的珠心上发芽。授以油松花粉的紅松,其胚珠发育至游离核时期即停止发育,与未授粉的紅松胚珠发育很相似。
Pinus koraiensis and Pinus tabulaeformis is not easy to get seeds. We try to find the reasons for the crossbred infertility by studying the development of large and small robe of Korean pine and Pinus tabulaeformis, the process of fertilization and the number of chromosomes. The test results are: 1. Korean pine from the pollination to fertilization interval of 12 months, pine is 13 months apart. After pollination, the pollen was stored in the pollen chamber at the top of the bead and started to germinate at an average temperature of about 22 ° C. Germinating pollen in winter pollen indoor, pollen tube next spring continue to stretch until mid-June fertilization. The number of chromosomes in P. korshinskii and P. tabulaeformis varied greatly, n = 6 in microspore mother cells and n = 12 in microspore mother cells; n = 6 in nobile cells and n = 7 in individual cells , 8, ..., 10, Pinus tabulaeformis endosperm cells are n = 12, there are n = 14. Due to the large difference in the number of chromosomes, it can also be deduced that it is difficult to cross between Pinus koraiensis and Pinus tabulaeformis. 3. The main reason for the mixed infertility of Pinus koraiensis and Pinus tabulaeformis is that pollen of Pinus tabulaeformis can not germinate on the heart of Pinus koraiensis. The Pinus tabulaeformis-fed Korean pine, its ovule development to the free nucleus that stopped development, and non-pollinated Pinus koraiensis ovule is very similar.