磁微球是以金属或金属氧化物为核,外面包被带有活性基团物质的一种新型生物分离材料.目前制备磁微球的方法有包埋法、聚合法、浸渍法、挤压法和生物合成法等.这种微球通过其活性基团与化学、生化和生物物质连接后,利用其顺磁性外加一定磁场可实现与介质分离.本文全面地介绍了磁微球的制备,详尽评述了其在免疫分析、核酸杂交分析、基因测序、细胞分离、酶的固定、受体分离等各个领域的应用. Magnetic microspheres is a new type of bio-separation material with metal or metal oxide as the core and coated with active groups, and the current methods for preparing magnetic microspheres include embedding, polymerization, dipping, extrusion Method and biosynthesis, etc. The microspheres can be separated from the medium by using its paramagnetic and magnetic field after its active groups are connected with the chemical, biochemical and biological substances through the active groups.This paper introduces the preparation of magnetic microspheres, Its application in various fields such as immunoassay, nucleic acid hybridization analysis, gene sequencing, cell separation, enzyme immobilization and receptor separation are reviewed in detail.