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目的:从6种蕨类植物中提取水溶活性物质。方法:利用滤纸片法对3种供试细菌(金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌)进行抑菌试验。结果:6种蕨类植物提取液对实验菌有不同程度的抑制作用,不同浓度的样品具有不同程度的抑菌作用,其中大芒萁提取液浓度为1.77%和栗柄金粉蕨提取液浓度为1.85%时对枯草芽孢杆菌的抑菌效果最强,抑菌圈直径均为16 mm。其他种蕨类植物提取液也均有抑菌效果。结论:6种蕨类植物均有抗菌物质存在,有望成为筛选抗菌新型药物的资源。
Objective: To extract water-soluble active substances from six kinds of ferns. Methods: Three kinds of test bacteria (Staphylococcus aureus, Bacillus subtilis and Escherichia coli) were tested for bacteriostasis by filter paper method. Results: Six kinds of fern extracts had different degrees of inhibitory effect on the experimental bacteria. The samples with different concentrations had antibacterial activity in different degrees. The concentration of the extract was 1.77% and the concentration of the extract was 1.85 Bacillus subtilis had the strongest bacteriostatic effect when the% was bacteriostatic, and the diameter of bacteriostatic circle was both 16 mm. Other fern extracts also have antibacterial effect. Conclusion: All 6 species of ferns have antimicrobial substances, which are expected to be the resources of screening new antimicrobial agents.