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本实验的目的旨在建立一种手术易行、结果可靠、重复性高的局限性缺血中风动物模型.实验用 SD 大鼠34只.动物麻醉后,应用显微外科手术经颅底入路阻断左侧大脑中动脉的起始段;手术24h 后,实验组中有27只大鼠出现不同程度的行为障碍.墨汁灌注发现这些大鼠的大部分左侧大脑皮质和基底神经节区域无血管或有少量血管显示,Nissle 和HE 染色发现此区域有典型的缺血性组织病理学改变.脑缺血区范围的大小与动物的行为障碍成正比.本实验为研究急性缺血性中风的病理机制提供了一个有用的实验动物模型.
The purpose of this experiment is to establish a surgical approach with reliable results and high repetitive ischemic stroke animal model.Experimental SD rats 34.Anesthesia, the application of microsurgical transcranial approach Blocking the initial segment of the left middle cerebral artery.After 24 hours of operation, 27 rats in the experimental group showed different degrees of behavioral disturbances.Most of the left cerebral cortex and basal ganglia region of these rats were found to be ink perfusion Blood vessels or a small number of blood vessels showed that Nissle and HE staining showed typical ischemic histopathological changes in this area.The size of the ischemic area was proportional to the behavioral disturbance in animals.This study was designed to investigate the effects of acute ischemic stroke Pathology provides a useful experimental animal model.