很多证据表明,研制血吸虫疫苗是现行控制血吸虫病策略的一个重要补充。致弱尾蚴诱导的明显的保护力主要针对幼虫期且与IgG抗体相关联。作者报告了用致弱尾蚴免疫兔血清IgG筛选曼氏血吸虫尾蚴cDNA文库以及1,6-二磷酸果糖醛缩酶的克隆、表达、免疫原性、酶活性测定及定位分析。 用~(60)Co照射致弱尾蚴腹膜下免疫新西兰兔,加强免疫两次取血清,用硫酸铵饱和沉淀 There is a lot of evidence that the development of schistosoma vaccine is an important complement to the current strategy to control schistosomiasis. The significant protection induced by attenuated cercariae is mainly directed to larval stages and is associated with IgG antibodies. The authors report the cloning, expression, immunogenicity, enzyme activity determination and localization analysis of Schistosoma mansoni cercaria cDNA library and fructose-1, 6-diphosphate dehydrogenase using rabbit serum immunized with weak cercariae. New Zealand rabbits were immunized subcutaneously with ~ (60) Co irradiation, and serum was twice harvested by immunization. Saturated sediment was precipitated with ammonium sulfate