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建立一套测定人指纹 ABO血型的方法体系。运用间接免疫酶技术和免疫印迹技术 ,对指纹胶纸提取的指纹、银粉显现提取的指纹、直接转移至硝酸纤维素膜 (NC膜 )上的指纹、银粉显现转移至 NC膜上的指纹、“5 0 2”胶熏显转移至 NC膜上的指纹等进行 ABO血型检测。上述 5种方法采集的 2 12枚指纹 ,绝大部分能正确地检出 ABO血型 ,检出率为 90 %～ 93.8% ;未能检出的样本为非分泌型人指纹。应用间接免疫酶技术及免疫印迹技术测定人指纹的 ABO血型的方法具有特异性强、准确可靠、检测方便等优点 ,在法庭科学中易推广应用 ,并能发挥重要的作用
To establish a set of methods for the determination of human fingerprinting ABO blood type system. Indirect immunoenzyme and Western blotting were used to fingerprint of extracted fingerprints and silver fingerprints. The fingerprints were directly transferred to the nitrocellulose membrane (NC membrane) and the silver particles were transferred to the fingerprints of NC membrane. 5 0 2 "smoked smoked significant transfer to the NC film on the fingerprints and other ABO blood group testing. Most of the 2 12 fingerprints collected by the above five methods can detect the ABO blood group correctly with the detection rate of 90% -93.8%. The samples that can not be detected are non-secret fingerprints. The method of using indirect immunoenzyme and immunoblotting to detect the ABO blood type of human fingerprints has the advantages of strong specificity, accuracy and reliability, convenient detection and so on, which is easy to popularize and apply in forensic science and plays an important role