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本实验对不同日龄雏麻鸭用含鸭乙型肝炎病毒(DHBV)鸭血清,经灌胃、静脉注射、肌肉注射等途径的人工感染,通过血清斑点杂交技术(~(32)P—DHBV DNA探针)、肝组织病理切片、电镜检查等方法,作动态观察,结果如下:①1～15d龄健康雏麻鸭94只,按日龄分组予DHBV灌胃后,经每周1次的反复采血检测,未发现血清DHBV DNA阳转者。②14d龄健康雏麻鸭30只,分组予静脉注射或肌内注射接种DHBV,1周后发现血清DHBV DNA阳转率分别为35.7％(5/15)和42.9％(6/15),2周后
In this study, DHBV ducks’ sera containing different ages of ducklings were inoculated intramuscularly, intravenously and intramuscularly by artificial infection. Serum dot blot hybridization (~ (32) P-DHBV DNA probes), pathological sections of liver tissue and electron microscopy. The results were as follows: (1) 94 healthy ducklings aged from 1 to 15 days were divided into groups according to their age and fed with DHBV once a week Blood tests, did not find serum DHBV DNA positive rotation. (2) Thirty healthy 14-year-old chickens were fed with intravenous injection or intramuscular injection of DHBV subcutaneously. After one week, DHBV DNA positive rates were 35.7% (5/15) and 42.9% (6/15) Rear