在缺氧和缺血性疾病中,机体能量代谢障碍的程度可用组织中高能核苷酸及其代谢物的含量变化来反映。3-硝基丙酸(3-NPA)是引起变质甘蔗中毒的主要毒素,它可抑制参与三羧酸循环的琥珀酸脱氢酶,使有氧氧化受阻而导致能量代谢障碍。本文参照Hull-Ryde方法建立了一个重复性好、灵敏度高的高能核苷酸及其代谢物的反相高效液相色谱测定方法,并将它用于3-NPA染毒大鼠大脑皮层中能 In hypoxic and ischemic diseases, the degree of energy metabolism disorders in the body can be reflected by changes in the content of high-energy nucleotides and their metabolites in tissues. 3-Nitropropionic acid (3-NPA) is the major toxin that causes sugarcane poisoning. It inhibits the succinate dehydrogenase involved in the tricarboxylic acid cycle, which blocks the oxidation of oxygen and leads to energy metabolism disorders. In this paper, a high reproducibility and high sensitivity high-energy nucleotide and its metabolite was determined by RP-HPLC method using Hull-Ryde method. The method was applied to the determination of 3-NPA in rat cerebral cortex