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毒性休克综合征(TSS)是由金黄色葡萄球菌感染伴发的一种多器官系统疾病,通常认为与毒性休克综合征毒素-1(TSST-1)有关。由TSST-1刺激单核细胞释放细胞因子的信号转导通道目前仍未确定。TSST-1似乎是通过几种细胞内磷蛋白脱磷酸作用而使单核细胞活化,但这种脱磷酸作用是否与细胞因子的诱导过程相联系,目前尚无定论。本文应用几种蛋白激酶抑制剂,在TSST-1刺激下,探讨了单核细胞和T淋巴细胞混合培养,分泌TNF-α和IL-1β的信号转导通道。实验结果表明,蛋白激酶C抑制剂(H_7)和酪氨酸激酶抑制剂(染料木黄酮)对TNF-α的分泌呈剂量依赖性抑制作用。根据台盼
Toxic shock syndrome (TSS) is a multiorgan system disease associated with Staphylococcus aureus infection and is commonly thought to be associated with toxic shock syndrome toxin-1 (TSST-1). The signal transduction pathway for the release of cytokines by monocytes stimulated by TSST-1 has not been established. TSST-1 appears to activate monocytes through the dephosphorylation of several intracellular phosphoproteins, but whether this dephosphorylation is linked to the induction of cytokines remains to be determined. In this paper, several protein kinase inhibitors were used to investigate the signal transduction pathways of TNF-α and IL-1β under the stimulation of TSST-1 in a mixed culture of monocytes and T lymphocytes. The experimental results show that protein kinase C inhibitor (H_7) and tyrosine kinase inhibitor (genistein) can inhibit the secretion of TNF-α in a dose-dependent manner. According to Taiwan hope