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目的采用硅胶表面分子印迹技术,制备印迹红霉素分子的聚合物材料,将其作为固相萃取介质,对自来水中添加的红霉素(erythromycin,EM)进行了富集纯化处理。方法制备中采用γ-氨丙基三甲氧基硅烷(3-aminopropyltrimethoxysilane,AMPS)作为硅胶(SiO2)改性剂,将功能单体甲基丙烯酸(methacrylic acid,MAA)接枝到AMPS-SiO2表面,形成聚甲基丙烯酸接枝硅胶(polymer methacrylic acid/SiO2,PMAA/SiO2)。最后,加入模板分子红霉素和交联剂乙二醇二甲基丙烯酸酯(2-ethanediol dimethacrylate,EDMA),聚合得到表面包被有红霉素印迹聚合物的硅胶材料(EMMIP-PMAA/SiO2)。通过电镜扫描(scanning electron microscope,SEM)和粒径测定对EM-MIPPMAA/SiO2物理性状进行了表征;通过动、静态吸附和选择性吸附试验研究了PMAA/SiO2和EMMIP-PMAA/SiO2对红霉素的结合性能和分子识别特性。结果 EM-MIP-PMAA/SiO2对红霉素显示了良好的吸附能力,最大吸附量可达104 mg·g-1,吸附约240 min达到吸附平衡;与竞争分子罗红霉素相比,表面印迹聚合物对模板分子红霉素显示了高的选择吸附能力;将其作为固相萃取介质对于生物样品中的分析物具有良好的纯化作用。结论像MIP-PMAA/SiO2这类新的印迹材料将会广泛应用于生物样品的监测分析工作,并且对于环境保护也具有一定实用意义和价值。
OBJECTIVE To prepare and purify erythromycin (EM) from tap water by using silica gel surface molecular imprinting technique to prepare polymer material of imprinted erythromycin molecule as a solid phase extraction medium. In the method, 3-aminopropyltrimethoxysilane (AMPS) was used as modifier of silica gel to graft methacrylic acid (MAA) onto AMPS-SiO2 surface. A polymethacrylic acid grafted silica gel (PMAA / SiO2) was formed. Finally, erythromycin (template molecule) and cross-linking agent ethylenedi methacrylate (EDMA) were added to obtain a silica gel material (EMMIP-PMAA / SiO2 ). The physical properties of EM-MIPPMAA / SiO2 were characterized by scanning electron microscope (SEM) and particle size measurement. The effects of PMAA / SiO2 and EMMIP-PMAA / SiO2 on the growth of erythromycin Succeeding binding properties and molecular recognition properties. Results EM-MIP-PMAA / SiO2 showed good adsorption capacity for erythromycin. The maximum adsorption capacity of EM-MIP-PMAA / SiO2 was 104 mg · g-1 and the adsorption equilibrium was reached at about 240 min. Compared with roxithromycin The imprinted polymer shows a high selective adsorption capacity to the template molecular erythromycin; it can be used as a solid phase extraction medium to purify analytes in biological samples. Conclusion New imprinting materials such as MIP-PMAA / SiO2 will be widely used in the monitoring and analysis of biological samples, and also have certain practical significance and value for environmental protection.