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鸟氨酸脱羧酶(ornithine decarboxylase,ODC)是催化多胺生物合成的限速酶.过去的研究表明多胺在维持肿瘤恶性表型中起着重要作用.用ODC不可逆抑制剂抑制多胺生物合成不仅能抑制肿瘤细胞的增殖而且还诱导细胞分化.近年我们采用反义技术,以ODC反义RNA转染肿瘤细胞,通过对ODC表达的封闭,成功地使其恶性表型得到了逆转.提示抑制肿瘤细胞的多胺合成可能是抗癌的有效途径.本文用ODC反义寡核苷酸控制肿瘤细胞的多胺生物合成,观察了其对K562细胞生长特性的影响,以期为ODC反义寡核苷酸在肿瘤临床的应用提供实验依据.
Ornithine decarboxylase (ODC) is a rate-limiting enzyme that catalyzes the biosynthesis of polyamines. Past studies have shown that polyamines play an important role in maintaining the malignant phenotype of tumors. Use of ODC irreversible inhibitors to inhibit polyamine biosynthesis Not only can inhibit the proliferation of tumor cells but also induce cell differentiation. In recent years, we used antisense technology to transfect the tumor cells with ODC antisense RNA, and successfully reversed the malignant phenotype by blocking the expression of ODC. The synthesis of polyamines in tumor cells may be an effective way to fight cancer. In this article, we used ODC antisense oligodeoxynucleotide to control the polyamine biosynthesis of tumor cells, and observed its effect on the growth characteristics of K562 cells, aiming at ODC antisense oligodeoxynucleotides. The use of glycosides in the clinical application of tumors provides experimental evidence.