本文研究了大豆抗花叶病毒病的鉴定方法。结果表明,毒源以采用干燥病叶在-15—-17℃条件下保存15天为宜,病叶与缓冲液比(g/V)为1:20。二片子叶展开时接种,其发病率较高,出现症状时间适宜。采用病株症状严重度及病叶量2个因素确定抗病5级标准,并以病情指数划分5个抗性等级。本研究提出的抗性鉴定方法可取得品种间及年份间具有可比性的抗性鉴定结果。 This article studies the identification of soybean anti-mosaic virus disease. The results showed that the source of poisonous leaves was preserved in dry leaves at -15-17 ℃ for 15 days, and the ratio of diseased leaves to buffer was 1:20. Two cotyledons spread when inoculated, the higher the incidence of symptoms of time appropriate. Disease severity and diseased leaves were used to determine the level of resistance to Disease 5 level five criteria, and the disease index is divided into five resistance levels. The resistance identification method proposed in this study can achieve the comparability of resistance identification between varieties and years.