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细胞介导的免疫应答是异种器官移植面临的主要障碍 ,细胞粘附是细胞间相互识别的第一步 .采用流式细胞术粘附测定法研究了人外周血单个核细胞 (PBMC)中单核细胞 (Mo)、自然杀伤细胞 (NK)和T淋巴细胞 (T)对猪主动脉内皮细胞 (PAEC)粘附的差异性 ,以及人细胞因子γ 干扰素 (hIFN γ)或 /和肿瘤坏死因子 α(hTNF α)预刺激PAEC 2 4h对PBMC不同亚群粘附的影响 .PBMC不同亚群对PAEC粘附能力的大小以Mo ,NK和T细胞为序 ,其中T细胞的粘附作用极其微弱 ;hTNF α具有种间交叉活性 ,可使Mo,NK和T细胞的粘附率分别提高40 % ,1 1 0 %和 3倍 .这些结果在细胞水平上直接证实了宿主Mo和NK细胞是异种移植细胞性排斥反应的主要参与者 ,为进一步研究人Mo/NK与PAEC间的作用机制打下了基础
Cell-mediated immune response is a major obstacle to xenotransplantation, and cell adhesion is the first step in cell-cell mutual recognition. Flow cytometry adhesion assay was used to study the effect of single-cell-peripheral blood mononuclear cells Differences in adhesion of nuclear, Moist and NK cells to porcine aortic endothelial cells (PAECs), as well as human cytokine interferon-gamma (IFN-γ) and / or tumor necrosis The effect of factor α (hTNF α) pretreatment on PAEC 2 4h adhesion to different subpopulations of PBMCs. The adhesion capacity of PAEC to different subpopulations of PBMCs was orderly Mo, NK and T cells, of which T cell adhesion was extremely Weak and hTNFα with inter-species cross-reactivity increased the adhesion rates of Mo, NK and T cells by 40%, 110% and 3 times respectively.These results directly confirmed that the host Mo and NK cells were The main participants in xenograft rejection were to lay the foundation for further study on the mechanism of action between human Mo / NK and PAEC