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目的探讨高脂血症患者肠道优势菌群变化及其与血清脂质水平的相关性。方法高脂血症患者及健康受试者各50例,采集其空腹血清样本和粪便样本,血清样本用于检测血清中总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)的含量。应用实时定量PCR技术检测肠道内优势菌群的含量,并将其与血清脂质水平进行相关性分析。结果高脂血症患者肠道内总细菌量及拟杆菌属细菌较健康受试者组差异无统计学意义(P>0.05),而双歧杆菌属细菌、乳杆菌属细菌及粪杆菌属细菌较健康受试者明显降低(P<0.05),肠杆菌科细菌和肠球菌属细菌较健康受试者明显升高(P<0.05)。高脂血症患者血清TC与双歧杆菌属细菌、乳杆菌属细菌和粪杆菌属细菌呈现显著负相关,而与肠杆菌科细菌和肠球菌属细菌呈现显著正相关;血清LDL-C与双歧杆菌属细菌和粪杆菌属细菌呈现显著负相关,而与肠球菌属细菌呈现显著正相关;血清TG与双歧杆菌属细菌和乳杆菌属细菌呈现显著负相关,而与肠杆菌科细菌和肠球菌属细菌呈现显著正相关。结论高脂血症患者肠道优势菌群发生了明显的变化,血清脂质水平与肠道优势菌群变化具有显著相关性,提示肠道优势菌群结构的调整可改善患者血清脂质水平。
Objective To investigate the changes of intestinal dominant bacteria in patients with hyperlipidemia and its correlation with serum lipid levels. Methods Fifty patients with hyperlipidemia and healthy subjects were enrolled in the study. Fasting serum samples and stool samples were collected. Serum samples were collected for determination of total cholesterol (TC), triglyceride (TG) and low density lipoprotein cholesterol LDL-C) content. Real-time quantitative PCR was used to detect the dominant intestinal microflora and its correlation with serum lipid level was analyzed. Results There was no significant difference in total intestinal bacteria and Bacteroides spp in hyperlipidemic patients compared with healthy subjects (P> 0.05). However, the bacteria in Bifidobacterium, Lactobacillus and Bacillus were more The healthy subjects significantly reduced (P <0.05), Enterobacteriaceae and Enterococcus bacteria significantly higher than the healthy subjects (P <0.05). Serum TC of patients with hyperlipemia was significantly negatively correlated with Bifidobacterium, Lactobacillus and Bacillus bacteria, but positively correlated with Enterobacteriaceae and Enterococcus bacteria. Serum LDL-C and There was a significant negative correlation between the bacteria of the genus Bacillus and that of the genus Bacillus, but a significant positive correlation with the bacteria of Enterococcus. The serum TG had a significant negative correlation with the bacteria of the genus Bifidobacterium and the bacteria of the genus Lactobacillus, Enterococcus bacteria showed a significant positive correlation. Conclusion The dominant intestinal microflora in patients with hyperlipidemia has obvious changes. The serum lipid level has a significant correlation with the dominant intestinal microflora. It suggests that the adjustment of dominant intestinal microflora may improve the serum lipid level.