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对金黄色葡萄球菌细胞结合胞外青霉素酶研究,找出了最有利于释放细胞结合胞外酶的条件,即以MIC/2量苯唑青霉素诱导和20%MgSO_4处理。在此条件下金黄色葡萄球菌菌株75释放的细胞结合胞外青霉素酶活力为8486 u/ml,是对照的19.87倍。用此条件证实26株耐药性金黄色葡萄球菌的细胞结合青霉素酶活力在19 937～382 033 u/ml;由此释出的部分细胞结合胞外青霉素酶活力在880～13 472u/ml。其中菌株85063释出的细胞结合胞外青霉素酶活力最高,为13 472u/ml。菌株85102的释出率最高,为6.23%。
For Staphylococcus aureus cells combined with extracellular penicillinase, the most favorable conditions for the release of cell-associated extracellular enzymes were found, ie, MIC / 2 amount of oxacillin induction and 20% MgSO 4 treatment. The cell-bound extracellular penicillinase activity of Staphylococcus aureus strain 75 released at this condition was 8486 u / ml, which is 19.87 times of the control. Using this condition, the cell-bound penicillinase activity of 26 drug-resistant Staphylococcus aureus was 19 937-38 2 033 u / ml. The cell-derived extracellular penicillinase activity of these 26 strains was 880-13 472 u / ml. Among them, strain 85063 released the highest cell-bound extracellular penicillinase activity of 13 472 u / ml. The highest release rate of strain 85102 was 6.23%.